• Controlled Ovarian Stimulation: During a normal menstrual cycle, a woman achieves the growth and development of a single follicle. Our objective through the administration of hormones is to produce a synchronous and controlled growth of several follicles in order to maximize the chances of success. Periodic visits will be made in order to evaluate ultrasound and hormonally the optimal moment for final maturation.

• Ovarian puncture: It is performed under sedation of the patient to guarantee maximum comfort during the procedure. With continuous transvaginal ultrasound guidance, the puncture and aspiration of each of the follicles that reached the optimal size is performed to be immediately passed on to our biologists in the laboratory.

• Fertilization of the oocytes: As decided by the medical team, the conventional technique or ICSI will be performed, with the partner's semen.  or from a donor who has been previously evaluated in our laboratory.

• Embryonic development in incubators: Our laboratory team maintains special surveillance of all the parameters that must be present for correct fertilization. We monitor 24 hours a day more than 10 parameters that directly influence the correct evolution of your embryos, in this way we guarantee the best embryo quality for transfer. After 24 hours of extraction, we already know the number of fertilized oocytes and we will continue to observe their evolution and development until the moment of their transfer, preferably at the blastocyst stage or day 5 post fertilization.

• embryo transfer: per protocol in Azul Fertility Experts All our patients should benefit from embryo transfer at the blastocyst stage (D5) since the embryos have a higher implantation rate.

Within an internationally established morphokinetic classification (ASRM, ASEBIR), we will always choose the best to be transferred. According to current legislation, a maximum of 1 to 2 embryos must be transferred. The selected embryos are visualized and preloaded in a fine cannula in the laboratory, the gynecologist visualizes the patient's neck and with ultrasound guidance passes a fine cannula to deposit the embryos in the most suitable place within the uterine cavity.

Each case must be individualized when transferring a certain number of embryos in order to minimize multiple pregnancies.

Cryopreservation: In Azul Fertility Experts We have a state-of-the-art embryo bank. Leftover embryos after a transfer can be preserved using a freezing technique called vitrification. These can be used later if gestation has not been achieved fresh or later when the patient so requires. We will explain to you during your consultation with the doctor what an endometrial preparation consists of for a previously frozen embryo transfer, which does not involve all the phases that we have described above.