In Vitro Fertilization

Photograph a Classic IVF obtained in the laboratory of Azul Fertility Experts.

In Vitro Fertilization

In Vitro Fertilization (IVF) is the most used and well-known technique in Assisted Reproduction, it consists of obtaining both gametes and putting them in contact in our laboratory (In Vitro) so that fertilization takes place.

During in vitro fertilization, we remove mature eggs from your ovaries, which are fertilized with sperm from your partner or a donor in our laboratory. Subsequently, the embryo is transferred to the uterus for gestation. A complete cycle of in vitro fertilization takes about three weeks. This process has different Phases.

This technique can generally be done in two different ways: 

classical IVF

In classic IVF, our biologists put each egg in contact with a number of sperm and they will unite spontaneously under optimal conditions monitored in the laboratory 24 hours a day.

video one Classic IVF obtained in the laboratory of Azul Fertility Experts.

Intracytoplasmic Sperm Injection or ICSI 

Micro Sperm Injection or ICSI is the other modality, it consists of manually selecting a very good quality sperm to be injected, with the help of a micromanipulator inside the mature oocyte.

Within the needs of each couple we can perform:

  • IVF with own eggs and partner's semen
  • IVF with own eggs and donor sperm
  • IVF with donor eggs and partner's sperm
  • IVF with donated eggs and sperm

video one Intracytoplasmic Sperm Injection or ICSI obtained in the laboratory of Azul Fertility Experts


Both Classic IVF and ICSI comprise the following phases:

  • Controlled Ovarian Stimulation: During a normal menstrual cycle, a woman achieves the growth and development of a single follicle. Our objective through the administration of hormones is to produce a synchronous and controlled growth of several follicles in order to maximize the chances of success. Periodic visits will be made in order to evaluate ultrasound and hormonally the optimal moment for final maturation.
  • Ovarian puncture: It is performed under sedation of the patient to guarantee maximum comfort during the procedure. With continuous transvaginal ultrasound guidance, the puncture and aspiration of each of the follicles that reached the optimal size is performed to be immediately passed on to our biologists in the laboratory.
  • Fertilization of the oocytes: As decided by the medical team, the conventional technique or ICSI will be performed, with the partner's semen.  or from a donor who has been previously evaluated in our laboratory.
  • Embryonic development in incubators: Our laboratory team maintains special surveillance of all the parameters that must be present for correct fertilization. We monitor 24 hours a day more than 10 parameters that directly influence the correct evolution of your embryos, in this way we guarantee the best embryo quality for transfer. After 24 hours of extraction, we already know the number of fertilized oocytes and we will continue to observe their evolution and development until the moment of their transfer, preferably at the blastocyst stage or day 5 post fertilization.
  • embryo transfer: per protocol in Azul Fertility Experts All our patients should benefit from embryo transfer at the blastocyst stage (D5) since the embryos have a higher implantation rate.

Within an internationally established morphokinetic classification (ASRM, ASEBIR), we will always choose the best to be transferred. According to current legislation, a maximum of 1 to 2 embryos must be transferred. The selected embryos are visualized and preloaded in a fine cannula in the laboratory, the gynecologist visualizes the patient's neck and with ultrasound guidance passes a fine cannula to deposit the embryos in the most suitable place within the uterine cavity.

Each case must be individualized when transferring a certain number of embryos in order to minimize multiple pregnancies.

Cryopreservation: In Azul Fertility Experts We have a state-of-the-art embryo bank. Leftover embryos after a transfer can be preserved using a freezing technique called vitrification. These can be used later if gestation has not been achieved fresh or later when the patient so requires. We will explain to you during your consultation with the doctor what an endometrial preparation consists of for a previously frozen embryo transfer, which does not involve all the phases that we have described above.

When is IVF recommended?


  • Absence, injury or obstruction of the fallopian tubes.
  • Endometriosis
  • Artificial Insemination Failure
  • Alterations in number, motility or sperm morphology.
  • Ovarian failure and decreased ovarian reserve
  • Ovulation disturbances
  • Advanced age
  • Infertility of unknown origin
  • in cancer patients.


Pregnancy rates will largely depend on the age of the patient at the time of the procedure. In patients under 35 years of age, on their first attempt the rates range from 60% to 20%-30% in patients over 40 years of age. These results are reported by our Medical Director in his extensive career, which in turn coincide with those reported by the American Association of Reproductive Medicine (ASRM) and European (ESHRE).

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