The method ROPA It is a method that is used in female-female homoparental families so that at the time of conceiving, both have participated in the process. One would be the carrier of the egg who assumes the ovarian stimulation to subsequently extract the eggs and fertilize them in the laboratory with donor sperm. The other woman will be the one who gestates the pregnancy with the ovules extracted from her partner.
The method ROPA starts with menstrual period timing of the couple Once they are already synchronized at the time we start ovarian stimulation, we will simultaneously be preparing the endometrium to achieve pregnancy.
Step by step of the method ROPA
Controlled Ovarian Stimulation: During a normal menstrual cycle, a woman achieves the growth and development of a single follicle. Our objective through the administration of hormones is to produce a synchronous and controlled growth of several follicles in order to maximize the chances of success. Periodic visits will be made in order to evaluate ultrasound and hormonally the optimal moment for final maturation. At the same time we will be evaluating the endometrial thickness of the couple in order to determine if their growth is optimal and what is expected for the moment of embryo transfer.
Ovarian puncture: It is done under sedation. With continuous transvaginal ultrasound guidance, the puncture and aspiration of each of the follicles that reached the optimal size to be passed immediately to our biologists in the laboratory is performed.
Fertilization of the oocytes: As decided by the medical team, the conventional or ICSI technique will be performed. The semen sample will come from our sperm bank from an anonymous donor, who has previously been evaluated and is suitable to be a donor> who has signed an informed consent for the donation. These pass a complex protocolized circuit of tests and interviews to finally be chosen. The samples are frozen in order to be certain of the window period of certain diseases.
Embryonic development in incubators: Our laboratory team maintains special surveillance of all the parameters that must be present for correct fertilization. After 24 hours of extraction we already have an idea of the number of fertilized oocytes, but it is obvious that they must be evaluated for their correct evolution and development.
embryo transfer: After 3 to 5 days, the pre-embryos are ready to be transferred to the patient's uterus, which has been preparing the endometrium Within an internationally established morphokinetic classification (ASRM, ASEBIR), we will always choose the best ones to be transferred. According to current legislation, a maximum of 1 to 2 embryos must be transferred. The selected embryos are visualized and preloaded in a fine cannula in the laboratory, the gynecologist visualizes the patient's neck and with ultrasound guidance passes a fine cannula to deposit the embryos in the most suitable place within the uterine cavity. The usual thing is to transfer a number of 1 to 2 embryos.
Cryopreservation: Today we have embryo banks in assisted reproduction units. Leftover embryos after a transfer can be preserved using a freezing technique called vitrification. These can be used later if gestation has not been achieved fresh or later when the patient so requires. Your doctor will explain to you what an endometrial preparation consists of, which does not involve all these phases that we have described.
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